Hotspot

trnI-GAU-rrn16

Genome · 111811-113217 · 1407 bp

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Pi 0.0272
MarkerSeek score 66.00
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
Genome
length_bp
1407
variable_sites
90
pi
0.0272
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.90
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 ACAACGACGCGATTATCAGG GTAGCTGTACTGGAAGGTGC 50.0 55.0 58.4 58.0 1248-1611 bp 1.0 85.4
trnI-GAU-rrn16_p2 ACAACGACGCGATTATCAGG GACCGGAGTGAAGTCGTAAC 50.0 55.0 58.4 58.0 1271-1634 bp 1.0 85.2
trnI-GAU-rrn16_p3 CACAACGACGCGATTATCAG GTAGCTGTACTGGAAGGTGC 50.0 55.0 57.4 58.0 1249-1612 bp 1.0 84.1
trnI-GAU-rrn16_p4 CACAACGACGCGATTATCAG GACCGGAGTGAAGTCGTAAC 50.0 55.0 57.4 58.0 1272-1635 bp 1.0 83.9
trnI-GAU-rrn16_p5 ACAACGACGCGATTATCAGG TGACCGGAGTGAAGTCGTAA 50.0 50.0 58.4 58.4 1272-1635 bp 1.0 82.3