Hotspot

chlN-ycf1

Genome · 81407-82513 · 1107 bp

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Pi 0.0142

MarkerSeek score 55.80

Primer available yes

Label chlN-ycf1

Feature Metadata

label_name: chlN-ycf1
feature_type: igs
region: Genome
length_bp: 1107
variable_sites: 36
pi: 0.0142
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-ycf1_p1	GAAGACTTGGATCGGACCAC	CCAATACGACATCATGGCCA	55.0	50.0	58.0	58.0	999-1379 bp	1.0	86.0
chlN-ycf1_p2	ACTTGGATCGGACCACCTTA	CCAATACGACATCATGGCCA	50.0	50.0	58.1	58.0	995-1375 bp	1.0	85.6
chlN-ycf1_p3	TTGGATCGGACCACCTTAGT	CCAATACGACATCATGGCCA	50.0	50.0	58.1	58.0	993-1373 bp	1.0	85.6
chlN-ycf1_p4	AGAAGACTTGGATCGGACCA	CCAATACGACATCATGGCCA	50.0	50.0	58.1	58.0	1000-1380 bp	1.0	85.6
chlN-ycf1_p5	CTTGGATCGGACCACCTTAG	CCAATACGACATCATGGCCA	55.0	50.0	57.4	58.0	994-1374 bp	1.0	81.2