Hotspot

rps4-trnQ-UUG

Genome · 63592-64142 · 551 bp

Back to Cephalotaxus

Pi 0.00807

MarkerSeek score 49.30

Primer available yes

Label rps4-trnQ-UUG

Feature Metadata

label_name: rps4-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 551
variable_sites: 16
pi: 0.00807
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnQ-UUG_p1	TTTTAAACGAGGTCCGCGAT	CCTTCGCAATAACAGGACCA	45.0	50.0	57.9	57.9	616-638 bp	1.0	82.8
rps4-trnQ-UUG_p2	TTTTAAACGAGGTCCGCGAT	ACCTTCGCAATAACAGGACC	45.0	50.0	57.9	57.9	617-639 bp	1.0	82.8
rps4-trnQ-UUG_p3	TTTTTAAACGAGGTCCGCGA	CCTTCGCAATAACAGGACCA	45.0	50.0	58.1	57.9	617-639 bp	1.0	82.4
rps4-trnQ-UUG_p4	TTTCTTGACAAGTCGCTCCC	CCTTCGCAATAACAGGACCA	50.0	50.0	58.1	57.9	694-716 bp	1.0	82.0
rps4-trnQ-UUG_p5	TTTCTTGACAAGTCGCTCCC	ACCTTCGCAATAACAGGACC	50.0	50.0	58.1	57.9	695-717 bp	1.0	82.0