Hotspot

trnE-UUC-trnT-GGU

Genome · 48792-49795 · 1004 bp

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Pi 0.00299
MarkerSeek score 49.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
Genome
length_bp
1004
variable_sites
8
pi
0.00299
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1099-1109 bp 1.0 79.9
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA TGAACACCGGGTTTTGTAGG 50.0 50.0 57.8 58.0 1148-1158 bp 1.0 79.7
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA CCTGAACACCGGGTTTTGTA 50.0 50.0 57.8 58.0 1150-1160 bp 1.0 79.6
trnE-UUC-trnT-GGU_p4 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 1078-1088 bp 1.0 79.5
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 1075-1085 bp 1.0 79.3