Hotspot

rps16

LSC · 4903-6089 · 1187 bp

Back to Cephalantheropsis

Pi 0.00758

MarkerSeek score 63.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1187
variable_sites: 9
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGTACTAGTTACCCTATCCTT	TCAATTGCATACGGTTCCAA	42.9	40.0	52.9	55.6	1275 bp	1.0	40.6
rps16_p2	GGTACTAGTTACCCTATCCTT	TGCATACGGTTCCAAATTCA	42.9	40.0	52.9	55.6	1270 bp	1.0	40.6
rps16_p3	GGTACTAGTTACCCTATCCTT	TGGTTCAGCCAATGACTATTCA	42.9	40.9	52.9	57.7	1312 bp	1.0	40.5
rps16_p4	GGTACTAGTTACCCTATCCTT	ATGTATCGATTGGTTCAGCC	42.9	45.0	52.9	55.6	1322 bp	1.0	40.5
rps16_p5	GGTACTAGTTACCCTATCCTTT	TGGTTCAGCCAATGACTATTCA	40.9	40.9	53.8	57.7	1312 bp	1.0	40.5