Hotspot

rpl33

LSC · 69151-69351 · 201 bp

Back to Cephalantheropsis

Pi 0.00498

MarkerSeek score 46.40

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 201
variable_sites: 1
pi: 0.00498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	TGGTTCGGATCAAGAAGAAA	ACTCTTCCCGCTACTTGGAA	40.0	50.0	54.6	58.4	314 bp	1.0	50.2
rpl33_p2	TTGGTTCGGATCAAGAAGAA	ACTCTTCCCGCTACTTGGAA	40.0	50.0	54.6	58.4	315 bp	1.0	50.2
rpl33_p3	TTTGGTTCGGATCAAGAAGA	ACTCTTCCCGCTACTTGGAA	40.0	50.0	54.6	58.4	316 bp	1.0	50.2
rpl33_p4	TGGTTCGGATCAAGAAGAAA	TCACACACACATTCCGTTCC	40.0	50.0	54.6	58.4	291 bp	1.0	49.8
rpl33_p5	TTGGTTCGGATCAAGAAGAA	TCACACACACATTCCGTTCC	40.0	50.0	54.6	58.4	292 bp	1.0	49.8