Hotspot

clpP

LSC · 71499-73792 · 2294 bp

Back to Cephalantheropsis

Pi 0.00174

MarkerSeek score 58.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2294
variable_sites: 4
pi: 0.00174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGGGTTGGCTTAGATCGATC	AACCCATTGTTACGTTTCCA	55.0	40.0	58.2	55.5	2451 bp	1.0	58.2
clpP_p2	CGGGTTGGCTTAGATCGATC	AAACCCATTGTTACGTTTCCA	55.0	38.1	58.2	56.3	2452 bp	1.0	56.6
clpP_p3	CGGGTTGGCTTAGATCGATC	ACCCATTGTTACGTTTCCAT	55.0	40.0	58.2	55.2	2450 bp	1.0	56.3
clpP_p4	CGGGTTGGCTTAGATCGATC	AACCCATTGTTACGTTTCCAT	55.0	38.1	58.2	56.0	2451 bp	1.0	54.8
clpP_p5	CGGGTTGGCTTAGATCGATC	AAAACCCATTGTTACGTTTCCA	55.0	36.4	58.2	57.0	2453 bp	1.0	54.4