Hotspot

psbB-psbT

LSC · 75995-76527 · 533 bp

Back to Cephalantheropsis

Pi 0.00375

MarkerSeek score 59.50

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 2
pi: 0.00375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	727 bp	1.0	66.9
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	726 bp	1.0	64.7
psbB-psbT_p3	GCCAGAACCTTGTTCAGAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	740 bp	1.0	64.6
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	689 bp	1.0	63.9
psbB-psbT_p5	GCCAGAACCTTGTTCAGAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	739 bp	1.0	62.4