Hotspot

trnD-GUC-trnY-GUA

LSC · 31634-31919 · 286 bp

Back to Cephalantheropsis

Pi 0.00699
MarkerSeek score 51.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
286
variable_sites
2
pi
0.00699
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 AATCAATTCGTCGGATCGGG CGAGATGTCTACGCTGGTTC 50.0 55.0 58.1 58.2 413 bp 1.0 77.9
trnD-GUC-trnY-GUA_p2 AATCAATTCGTCGGATCGGG GGGGACGGACTGTAAATTCG 50.0 55.0 58.1 58.4 437 bp 1.0 76.5
trnD-GUC-trnY-GUA_p3 AATCAATTCGTCGGATCGGG GGGACGGACTGTAAATTCGT 50.0 50.0 58.1 57.6 436 bp 1.0 76.3
trnD-GUC-trnY-GUA_p4 AATCAATTCGTCGGATCGGG CGGACTGTAAATTCGTTGGC 50.0 50.0 58.1 57.5 432 bp 1.0 75.1
trnD-GUC-trnY-GUA_p5 AATCAATTCGTCGGATCGGG GGACTGTAAATTCGTTGGCG 50.0 50.0 58.1 57.5 431 bp 1.0 75.1