Hotspot

petN-psbM

LSC · 29558-30349 · 792 bp

Back to Cephalantheropsis

Pi 0.00253

MarkerSeek score 60.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 2
pi: 0.00253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGACATCATTCGCATGGAT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.1	57.9	986 bp	1.0	78.6
petN-psbM_p2	GTCTCACTTGGGCTGCTTTA	CTTGCGCTTATTGCTACTGC	50.0	50.0	57.8	57.9	959 bp	1.0	77.9
petN-psbM_p3	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.1	58.3	925 bp	1.0	77.3
petN-psbM_p4	GGGACATCATTCGCATGGAT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.1	58.3	977 bp	1.0	77.3
petN-psbM_p5	ACTTGGGCTGCTTTAATGGT	CTTGCGCTTATTGCTACTGC	45.0	50.0	57.7	57.9	954 bp	1.0	76.9