Hotspot

trnC-GCA-petN

LSC · 28681-29461 · 781 bp

Back to Cephalantheropsis

Pi 0.00256

MarkerSeek score 60.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 2
pi: 0.00256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCAGTTCGAATCTGGGTGTC	TAAAGCAGCCCAAGTGAGAC	55.0	50.0	58.3	57.8	854-871 bp	1.0	76.5
trnC-GCA-petN_p2	CCAGTTCGAATCTGGGTGTC	ACCATTAAAGCAGCCCAAGT	55.0	45.0	58.3	57.7	859-876 bp	1.0	75.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	TAAAGCAGCCCAAGTGAGAC	50.0	50.0	57.5	57.8	877-894 bp	1.0	74.5
trnC-GCA-petN_p4	CCAGTTCGAATCTGGGTGTC	GCAGCCCAAGTGAGACTTAC	55.0	55.0	58.3	58.6	850-867 bp	1.0	73.6
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	ACCATTAAAGCAGCCCAAGT	50.0	45.0	57.5	57.7	882-899 bp	1.0	73.5