Hotspot

atpI-rps2

LSC · 15586-15882 · 297 bp

Back to Cephalantheropsis

Pi 0.00673

MarkerSeek score 56.40

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 2
pi: 0.00673
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	TGGCCTACTTCTACACCTGA	TGTGAGGGCCGTTCTAGTTA	50.0	50.0	57.4	58.1	404 bp	1.0	74.9
atpI-rps2_p2	GCTGGCCTACTTCTACACCT	TGTGAGGGCCGTTCTAGTTA	55.0	50.0	58.8	58.1	406 bp	1.0	73.4
atpI-rps2_p3	TGGCCTACTTCTACACCTGA	ATTTGTGAGGGCCGTTCTAG	50.0	50.0	57.4	57.6	407 bp	1.0	72.2
atpI-rps2_p4	CTGGCCTACTTCTACACCTG	TGTGAGGGCCGTTCTAGTTA	55.0	50.0	57.0	58.1	405 bp	1.0	72.1
atpI-rps2_p5	ATGCTGGCCTACTTCTACAC	TGTGAGGGCCGTTCTAGTTA	50.0	50.0	57.0	58.1	408 bp	1.0	71.9