Hotspot

rpl32-ndhF

SSC · 131216-132541 · 1326 bp

Back to Cephalanthera

Pi 0.0182

MarkerSeek score 72.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1326
variable_sites: 57
pi: 0.0182
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTTACTGCCGCTAAAGAGCC	GGAGCGGAAAAAGGGGTATT	50.0	50.0	58.3	57.9	1456-1537 bp	1.0	83.8
rpl32-ndhF_p2	TTTACTGCCGCTAAAGAGCC	ACATAGTAACTGGGAGCGGA	50.0	50.0	58.3	57.8	1468-1549 bp	1.0	83.7
rpl32-ndhF_p3	TTTACTGCCGCTAAAGAGCC	TAACTGGGAGCGGAAAAAGG	50.0	50.0	58.3	57.8	1462-1543 bp	1.0	83.4
rpl32-ndhF_p4	TGCCGCTAAAGAGCCTTTTT	GGAGCGGAAAAAGGGGTATT	45.0	50.0	58.4	57.9	1451-1532 bp	1.0	82.9
rpl32-ndhF_p5	TGCCGCTAAAGAGCCTTTTT	ACATAGTAACTGGGAGCGGA	45.0	50.0	58.4	57.8	1463-1544 bp	1.0	82.7