Hotspot

ndhE-psaC

SSC · 126233-127067 · 835 bp

Back to Cephalanthera

Pi 0.0161

MarkerSeek score 63.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 835
variable_sites: 35
pi: 0.0161
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TCGTAACAGAAAATCAACTCGT	AGCTCGAACACATTGGGTAC	36.4	50.0	56.1	57.9	947-1062 bp	1.0	51.9
ndhE-psaC_p2	TCGTAACAGAAAATCAACTCGT	ACATCCGTCCCAAGGTATCA	36.4	50.0	56.1	58.1	989-1104 bp	1.0	51.5
ndhE-psaC_p3	TCGTAACAGAAAATCAACTCGT	TCGAACACATTGGGTACACC	36.4	50.0	56.1	57.8	944-1059 bp	1.0	51.3
ndhE-psaC_p4	TCGTAACAGAAAATCAACTCGT	GCTCGAACACATTGGGTACA	36.4	50.0	56.1	58.2	946-1061 bp	1.0	51.1
ndhE-psaC_p5	CATCGTAACAGAAAATCAACTCGT	AGCTCGAACACATTGGGTAC	37.5	50.0	57.8	57.9	949-1064 bp	1.0	50.1