Hotspot

rps8-rpl14

LSC · 85126-85404 · 279 bp

Back to Cephalanthera

Pi 0.0346

MarkerSeek score 77.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 21
pi: 0.0346
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	CGATTGCTCGGGAATTGAGA	55.0	50.0	58.1	58.1	369-408 bp	1.0	84.8
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	TTGCTCGGGAATTGAGACAG	55.0	50.0	58.1	57.9	366-405 bp	1.0	84.6
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.1	57.9	364-403 bp	1.0	84.6
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	CGAATTTTTGGTGCGATTGC	55.0	45.0	58.1	57.2	382-421 bp	1.0	79.0
rps8-rpl14_p5	CGGCAATAATGTCCCTACCC	ATTGCTCGGGAATTGAGACA	55.0	45.0	58.1	57.2	367-406 bp	1.0	79.0