Hotspot

psbT-psbN

LSC · 78674-78743 · 70 bp

Back to Cephalanthera

Pi 0.061

MarkerSeek score 64.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 8
pi: 0.061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	CCTTCGAAGAACATGGGGAC	47.6	55.0	57.6	58.3	132 bp	1.0	71.6
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	CCCTAGTCGCCATCTCCATA	47.6	55.0	57.6	58.1	228 bp	1.0	71.0
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	184 bp	1.0	70.0
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	CCTTCGAAGAACATGGGGAC	47.6	55.0	57.3	58.3	133 bp	1.0	69.3
psbT-psbN_p5	TCGAGAACCACCTAAAGTTCC	CCCTAGTCGCCATCTCCATA	47.6	55.0	57.3	58.1	229 bp	1.0	68.8