Hotspot

psbB-psbT

LSC · 78230-78565 · 336 bp

Back to Cephalanthera

Pi 0.0177

MarkerSeek score 64.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 14
pi: 0.0177
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	435-558 bp	1.0	72.8
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	434-557 bp	1.0	70.6
psbB-psbT_p3	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	397-520 bp	1.0	70.0
psbB-psbT_p4	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGAA	45.0	45.5	57.7	58.0	434-557 bp	1.0	68.0
psbB-psbT_p5	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	396-519 bp	1.0	67.8