Hotspot

clpP-psbB

LSC · 75944-76702 · 759 bp

Back to Cephalanthera

Pi 0.0157

MarkerSeek score 72.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 28
pi: 0.0157
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	937-1221 bp	1.0	83.4
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	932-1216 bp	1.0	83.2
clpP-psbB_p3	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	903-1187 bp	1.0	82.9
clpP-psbB_p4	TATACGTCAACCCAAGCTGC	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	898-1182 bp	1.0	82.8
clpP-psbB_p5	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	855-1139 bp	1.0	82.7