Hotspot

trnW-CCA-trnP-UGG

LSC · 70023-70189 · 167 bp

Back to Cephalanthera

Pi 0.0253

MarkerSeek score 65.00

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 12
pi: 0.0253
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ATTTGAACCTACGACGTCGG	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	255-256 bp	1.0	83.1
trnW-CCA-trnP-UGG_p2	ATTTGAACCTACGACGTCGG	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	253-254 bp	1.0	83.1
trnW-CCA-trnP-UGG_p3	ATTTGAACCTACGACGTCGG	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	256-257 bp	1.0	83.1
trnW-CCA-trnP-UGG_p4	ATTTGAACCTACGACGTCGG	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	266-267 bp	1.0	83.0
trnW-CCA-trnP-UGG_p5	ATTTGAACCTACGACGTCGG	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	267-268 bp	1.0	83.0