Hotspot

psbE-petL

LSC · 68146-69432 · 1287 bp

Back to Cephalanthera

Pi 0.00951
MarkerSeek score 64.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1287
variable_sites
32
pi
0.00951
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCTGGGGTTATAGTTGAAGCA 50.0 47.6 57.9 58.0 1436-1452 bp 1.0 74.6
psbE-petL_p2 CAACCCGCAATGAATAGGGA GGGTTATAGTTGAAGCAGCCA 50.0 47.6 57.9 58.0 1432-1448 bp 1.0 74.6
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1434-1450 bp 1.0 74.6
psbE-petL_p4 ACTGACGAATAACCAACCCG GGGTTATAGTTGAAGCAGCCA 50.0 47.6 57.9 58.0 1445-1461 bp 1.0 74.3
psbE-petL_p5 ACTGACGAATAACCAACCCG TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1447-1463 bp 1.0 74.3