Hotspot

accD-psaI

LSC · 61699-62560 · 862 bp

Back to Cephalanthera

Pi 0.0136

MarkerSeek score 58.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 24
pi: 0.0136
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	717-1092 bp	1.0	85.9
accD-psaI_p2	AATGCCTACATTGCGTTTGC	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.0	58.0	786-1161 bp	1.0	85.8
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	849-1224 bp	1.0	85.7
accD-psaI_p4	AATGCCTACATTGCGTTTGC	TTGCTGAGACGGAAATACGG	45.0	50.0	58.0	58.0	1367-1742 bp	1.0	85.3
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TTGCTGAGACGGAAATACGG	55.0	50.0	58.0	58.0	1430-1805 bp	1.0	85.3