Hotspot

trnK-UUU-rps16

LSC · 4223-4937 · 715 bp

Back to Cephalanthera

Pi 0.0166

MarkerSeek score 61.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 34
pi: 0.0166
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CACATCCGTCTCCAATCCAG	GCAAAGACAACGAATTCCGG	55.0	50.0	58.1	58.0	1856-1866 bp	1.0	82.4
trnK-UUU-rps16_p2	TAAGAAGGGAAGCTCGTTGC	GCAAAGACAACGAATTCCGG	50.0	50.0	57.9	58.0	1699-1709 bp	1.0	82.3
trnK-UUU-rps16_p3	CACATCCGTCTCCAATCCAG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	58.1	57.9	1446-1454 bp	1.0	82.0
trnK-UUU-rps16_p4	ACATCCGTCTCCAATCCAGA	GCAAAGACAACGAATTCCGG	50.0	50.0	58.1	58.0	1855-1865 bp	1.0	81.8
trnK-UUU-rps16_p5	CTTTTCCCACATCAGGCACT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.1	58.0	1772-1782 bp	0.833333	75.3