Hotspot

rpoC2

LSC · 42397-46560 · 4164 bp

Back to Centrosema

Pi 0.0065

MarkerSeek score 46.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4164
variable_sites: 27
pi: 0.0065
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGAGAAATGCCGATCTTTGA	AATTTTCTCGTTTTCGTCCA	40.0	35.0	55.1	53.8	304 bp	1.0	45.3
rpoC2_p2	TGAGAAATGCCGATCTTTGA	TTTTCTCGTTTTCGTCCATT	40.0	35.0	55.1	53.8	302 bp	1.0	45.3
rpoC2_p3	TGAGAAATGCCGATCTTTGA	ATTTTCTCGTTTTCGTCCAT	40.0	35.0	55.1	53.5	303 bp	1.0	45.3
rpoC2_p4	TGAGAAATGCCGATCTTTGA	ATTTTCTCGTTTTCGTCCATT	40.0	33.3	55.1	54.4	303 bp	1.0	45.3
rpoC2_p5	TGAGAAATGCCGATCTTTGA	AAATTTTCTCGTTTTCGTCCA	40.0	33.3	55.1	54.6	305 bp	1.0	45.2