Hotspot

rpl16

LSC · 81890-83723 · 1834 bp

Back to Centrosema

Pi 0.0193

MarkerSeek score 59.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1834
variable_sites: 34
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCTCGGGTGCTAATGAAAC	ATTAACTGAACAAGCGGGCA	50.0	45.0	57.9	58.1	2673-2684 bp	1.0	81.8
rpl16_p2	ACCTCGGGTGCTAATGAAAC	ATCTTCTGGAGTCGAGGGAG	50.0	55.0	57.9	57.9	2967-2978 bp	1.0	81.7
rpl16_p3	GCGCAAAAGATTCGAGTTCC	ATTAACTGAACAAGCGGGCA	50.0	45.0	58.1	58.1	2613-2624 bp	1.0	81.7
rpl16_p4	GCGCAAAAGATTCGAGTTCC	ATCTTCTGGAGTCGAGGGAG	50.0	55.0	58.1	57.9	2907-2918 bp	1.0	81.6
rpl16_p5	ACGATTGCGCAAAAGATTCG	ATCTTCTGGAGTCGAGGGAG	45.0	55.0	58.1	57.9	2913-2924 bp	1.0	81.6