Hotspot

ndhD-psaC

SSC · 117029-117161 · 133 bp

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Pi 0.0301

MarkerSeek score 64.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 133
variable_sites: 4
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAAATTAAAGAACCGCCAACT	AAACAACTCGAAGCATGGGT	34.8	45.0	57.2	58.0	232 bp	1.0	51.0
ndhD-psaC_p2	AGAAAATTAAAGAACCGCCAACT	GGCATGAAACAACTCGAAGC	34.8	50.0	57.2	58.0	238 bp	1.0	50.8
ndhD-psaC_p3	AGAAAATTAAAGAACCGCCAACT	AGCATGGGTCTCGCTTATTG	34.8	50.0	57.2	58.0	221 bp	1.0	50.8
ndhD-psaC_p4	AGAAAATTAAAGAACCGCCAACT	GCATGGGTCTCGCTTATTGA	34.8	50.0	57.2	58.0	220 bp	1.0	50.8
ndhD-psaC_p5	AGAAAATTAAAGAACCGCCAACT	GAAGCATGGGTCTCGCTTAT	34.8	50.0	57.2	57.8	223 bp	1.0	49.2