Hotspot

ndhF-rpl32

SSC · 112686-113459 · 774 bp

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Pi 0.0199

MarkerSeek score 59.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 774
variable_sites: 14
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GATTTAGCGACTGAGCCACA	GTACGCTTTTTGGGAACTGC	50.0	50.0	58.0	58.0	1512-1519 bp	1.0	84.3
ndhF-rpl32_p2	GATTTAGCGACTGAGCCACA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	2227-2245 bp	1.0	83.7
ndhF-rpl32_p3	TAGCAAGAGGGCACACAAAG	GTACGCTTTTTGGGAACTGC	50.0	50.0	58.1	58.0	1489-1496 bp	1.0	83.6
ndhF-rpl32_p4	GATTTAGCGACTGAGCCACA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	2220-2238 bp	1.0	83.2
ndhF-rpl32_p5	GATTTAGCGACTGAGCCACA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	2225-2243 bp	1.0	83.1