Hotspot

rpl20-rps12

LSC · 68745-69583 · 839 bp

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Pi 0.0085
MarkerSeek score 65.30
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
839
variable_sites
7
pi
0.0085
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 GTCCTTCGTTTCCGAGCTAT ACAGCCAATCCGAAATGTCA 50.0 45.0 57.4 57.8 949-962 bp 1.0 75.6
rpl20-rps12_p2 GTCCTTCGTTTCCGAGCTAT ACAAGACAGCCAATCCGAAA 50.0 45.0 57.4 57.7 954-967 bp 1.0 75.0
rpl20-rps12_p3 TAAGTCTTGAATGAGCCCCG ACAGCCAATCCGAAATGTCA 50.0 45.0 57.3 57.8 999-1012 bp 1.0 74.6
rpl20-rps12_p4 TGTCCTTCGTTTCCGAGCTA ACAGCCAATCCGAAATGTCA 50.0 45.0 58.7 57.8 950-963 bp 1.0 74.3
rpl20-rps12_p5 TAAGTCTTGAATGAGCCCCG ACAAGACAGCCAATCCGAAA 50.0 45.0 57.3 57.7 1004-1017 bp 1.0 74.0