Hotspot

trnP-UGG-psaJ

LSC · 66459-66903 · 445 bp

Back to Centrosema

Pi 0.0253

MarkerSeek score 61.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 10
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	ACTACTGGTGCCACTGAAAG	50.0	50.0	58.1	57.4	491-541 bp	1.0	80.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	ACTACTGGTGCCACTGAAAG	50.0	50.0	58.1	57.4	490-540 bp	1.0	80.3
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	ACTACTGGTGCCACTGAAAG	50.0	50.0	58.1	57.4	502-552 bp	1.0	80.2
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	ACTACTGGTGCCACTGAAAG	50.0	50.0	58.1	57.4	501-551 bp	1.0	80.2
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	ACTACTGGTGCCACTGAAAG	50.0	50.0	58.1	57.4	504-554 bp	1.0	80.2