Hotspot

psaI-cemA

LSC · 59614-60871 · 1258 bp

Back to Centrosema

Pi 0.0245

MarkerSeek score 69.20

Primer available yes

Label psaI-cemA

Feature Metadata

label_name: psaI-cemA
feature_type: igs
region: LSC
length_bp: 1258
variable_sites: 30
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-cemA_p1	ATTTCCGGCAATTGCAATGG	TGTGAAAGAGATGCACCAGG	45.0	50.0	58.0	57.8	1371-1397 bp	1.0	81.9
psaI-cemA_p2	GGCAATTGCAATGGCTTCTT	TGTGAAAGAGATGCACCAGG	45.0	50.0	57.9	57.8	1365-1391 bp	1.0	81.3
psaI-cemA_p3	GGCCTAGTATTTCCGGCAAT	TGTGAAAGAGATGCACCAGG	50.0	50.0	57.7	57.8	1379-1405 bp	1.0	79.9
psaI-cemA_p4	ATTTCCGGCAATTGCAATGG	GTGAAAGAGATGCACCAGGG	45.0	55.0	58.0	58.5	1370-1396 bp	1.0	79.0
psaI-cemA_p5	GGCAATTGCAATGGCTTCTT	GTGAAAGAGATGCACCAGGG	45.0	55.0	57.9	58.5	1364-1390 bp	1.0	78.4