Hotspot

trnT-GGU-trnE-UUC

LSC · 30730-31546 · 817 bp

Back to Centrosema

Pi 0.011

MarkerSeek score 65.10

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 9
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	958-973 bp	1.0	79.4
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	TTTCGTAGTACCCTACCCCC	55.0	55.0	58.1	57.8	937-952 bp	1.0	79.0
trnT-GGU-trnE-UUC_p3	TGGCGTTACTCTACCACTGA	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.1	57.8	934-949 bp	1.0	78.8
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	920-935 bp	1.0	78.6
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	899-914 bp	1.0	78.5