Hotspot

trnS-UGA-psbC

LSC · 26640-26865 · 226 bp

Back to Centrosema

Pi 0.0442

MarkerSeek score 70.40

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 10
pi: 0.0442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.0	58.4	425-429 bp	1.0	78.3
trnS-UGA-psbC_p2	ATAGCCCCGGTCTTGAAAAC	TAGGTCATTTATGGCACGCG	50.0	50.0	57.9	58.4	405-409 bp	1.0	77.6
trnS-UGA-psbC_p3	GCTTTTGGAGAGATGGCTGA	TAGGTCATTTATGGCACGCG	50.0	50.0	57.9	58.4	432-436 bp	1.0	77.5
trnS-UGA-psbC_p4	GGGTTCGAATCCCTCTCTCT	TAGGTCATTTATGGCACGCG	55.0	50.0	58.2	58.4	356-360 bp	1.0	77.1
trnS-UGA-psbC_p5	ATGGCTGAGTGGTTGATAGC	TAGGTCATTTATGGCACGCG	50.0	50.0	57.7	58.4	420-424 bp	1.0	75.8