Hotspot

psbZ-trnS-UGA

LSC · 26139-26546 · 408 bp

Back to Centrosema

Pi 0.0104

MarkerSeek score 58.90

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 4
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CGACCAACCATCAGGAGAAG	CAACCACTCAGCCATCTCTC	55.0	55.0	58.0	58.0	511-531 bp	1.0	83.7
psbZ-trnS-UGA_p2	CGACCAACCATCAGGAGAAG	GTTTTCAAGACCGGGGCTAT	55.0	50.0	58.0	57.9	531-551 bp	1.0	82.7
psbZ-trnS-UGA_p3	CCAACCATCAGGAGAAGCAA	CAACCACTCAGCCATCTCTC	50.0	55.0	57.8	58.0	508-528 bp	1.0	82.2
psbZ-trnS-UGA_p4	TACTCGACCAACCATCAGGA	CAACCACTCAGCCATCTCTC	50.0	55.0	57.8	58.0	515-535 bp	1.0	82.0
psbZ-trnS-UGA_p5	GACCAACCATCAGGAGAAGC	CAACCACTCAGCCATCTCTC	55.0	55.0	58.3	58.0	510-530 bp	1.0	81.8