Hotspot

trnL (UAG)-rpl32

SSC · 124854-125325 · 472 bp

Back to Centropogon

Pi 0.0228
MarkerSeek score 59.40
Primer available yes
Label trnL (UAG)-rpl32

Feature Metadata

label_name
trnL (UAG)-rpl32
feature_type
igs
region
SSC
length_bp
472
variable_sites
10
pi
0.0228
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL (UAG)-rpl32_p1 AGCGTGTCTACCGATTTCAC GGAAGGGATATTCATCGGCG 50.0 55.0 58.0 58.3 584-599 bp 1.0 83.4
trnL (UAG)-rpl32_p2 CAGCGTGTCTACCGATTTCA GGAAGGGATATTCATCGGCG 50.0 55.0 58.0 58.3 585-600 bp 1.0 83.4
trnL (UAG)-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GGAAGGGATATTCATCGGCG 50.0 55.0 57.9 58.3 594-609 bp 1.0 82.4
trnL (UAG)-rpl32_p4 GAAGTAGCAAAGCCTTGGGT GGAAGGGATATTCATCGGCG 50.0 55.0 58.1 58.3 1077-1092 bp 1.0 80.8
trnL (UAG)-rpl32_p5 TGAAGTAGCAAAGCCTTGGG GGAAGGGATATTCATCGGCG 50.0 55.0 58.1 58.3 1078-1093 bp 1.0 80.8