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trnT (UGU)-trnL (UAA)

LSC · 52903-53618 · 716 bp

Back to Centropogon

Pi 0.0251

MarkerSeek score 71.10

Primer available yes

Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name: trnT (UGU)-trnL (UAA)
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 17
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT (UGU)-trnL (UAA)_p1	GCGATGCTCTACCTTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	728-766 bp	1.0	84.7
trnT (UGU)-trnL (UAA)_p2	GCGATGCTCTACCTTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	736-774 bp	1.0	84.2
trnT (UGU)-trnL (UAA)_p3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	760-798 bp	1.0	83.8
trnT (UGU)-trnL (UAA)_p4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	768-806 bp	1.0	83.2
trnT (UGU)-trnL (UAA)_p5	GCGATGCTCTACCTTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	742-780 bp	1.0	82.8