Hotspot

trnS (GCU)-trnG (UCC)

LSC · 14055-14774 · 720 bp

Back to Centropogon

Pi 0.0139

MarkerSeek score 66.70

Primer available yes

Label trnS (GCU)-trnG (UCC)

Feature Metadata

label_name: trnS (GCU)-trnG (UCC)
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 10
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS (GCU)-trnG (UCC)_p1	ACGGAAAGAGAGGGATTCGA	ACCGAACGACCCCTTAACTA	50.0	50.0	58.2	58.1	880 bp	1.0	79.1
trnS (GCU)-trnG (UCC)_p2	ACGAAAACGGAAAGAGAGGG	ACCGAACGACCCCTTAACTA	50.0	50.0	57.8	58.1	886 bp	1.0	79.0
trnS (GCU)-trnG (UCC)_p3	ACGGAAAGAGAGGGATTCGA	AAACCGAACGACCCCTTAAC	50.0	50.0	58.2	58.1	882 bp	1.0	78.8
trnS (GCU)-trnG (UCC)_p4	ACGAAAACGGAAAGAGAGGG	AAACCGAACGACCCCTTAAC	50.0	50.0	57.8	58.1	888 bp	1.0	78.6
trnS (GCU)-trnG (UCC)_p5	AGTCATCGACGAAAACGGAA	ACCGAACGACCCCTTAACTA	45.0	50.0	57.6	58.1	894 bp	1.0	76.9