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matK-trnK (UUU)

LSC · 9990-10720 · 731 bp

Back to Centropogon

Pi 0.00414

MarkerSeek score 62.10

Primer available yes

Label matK-trnK (UUU)

Feature Metadata

label_name: matK-trnK (UUU)
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 3
pi: 0.00414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK (UUU)_p1	CCATGATCATGAGCAAGTGC	TATGCTGTGATTCGCTCGTC	50.0	50.0	57.2	58.2	938-946 bp	1.0	73.4
matK-trnK (UUU)_p2	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.2	58.2	855-862 bp	1.0	73.4
matK-trnK (UUU)_p3	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	854-861 bp	1.0	73.4
matK-trnK (UUU)_p4	CCATGATCATGAGCAAGTGC	TTATGCTGTGATTCGCTCGT	50.0	45.0	57.2	57.7	939-947 bp	1.0	72.4
matK-trnK (UUU)_p5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.2	57.5	868-875 bp	1.0	71.0