Hotspot

psbA-rbcL

LSC · 1480-2556 · 1077 bp

Back to Centropogon

Pi 0.0102

MarkerSeek score 64.60

Primer available yes

Label psbA-rbcL

Feature Metadata

label_name: psbA-rbcL
feature_type: igs
region: LSC
length_bp: 1077
variable_sites: 11
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rbcL_p1	TACAGAAGCGACCCCATAGG	ATTTGAGTTTGCCGCAATGG	55.0	45.0	58.6	57.9	1163 bp	1.0	75.4
psbA-rbcL_p2	TACAGAAGCGACCCCATAGG	GCTGCTTGTGAGGTATGGAA	55.0	50.0	58.6	57.9	1192 bp	1.0	75.1
psbA-rbcL_p3	TACAGAAGCGACCCCATAGG	TGAGTTTGCCGCAATGGATA	55.0	45.0	58.6	57.9	1160 bp	1.0	75.1
psbA-rbcL_p4	TACAGAAGCGACCCCATAGG	AGTTTGCCGCAATGGATACT	55.0	45.0	58.6	57.9	1158 bp	1.0	75.0
psbA-rbcL_p5	ATCCAGTTACAGAAGCGACC	ATTTGAGTTTGCCGCAATGG	50.0	45.0	57.3	57.9	1170 bp	1.0	74.7