Hotspot

trnH (GUG)-psbA

LSC · 78-417 · 340 bp

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Pi 0.0239
MarkerSeek score 65.10
Primer available yes
Label trnH (GUG)-psbA

Feature Metadata

label_name
trnH (GUG)-psbA
feature_type
igs
region
LSC
length_bp
340
variable_sites
8
pi
0.0239
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH (GUG)-psbA_p1 CAATCCACTGCCTTCATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 468-473 bp 1.0 78.5
trnH (GUG)-psbA_p2 AATCCACTGCCTTCATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 467-472 bp 1.0 78.4
trnH (GUG)-psbA_p3 ACAATCCACTGCCTTCATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 469-474 bp 1.0 78.4
trnH (GUG)-psbA_p4 CAATCCACTGCCTTCATCCA ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 469-474 bp 1.0 78.0
trnH (GUG)-psbA_p5 AATCCACTGCCTTCATCCAC ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 468-473 bp 1.0 78.0