Hotspot

rpoC2

LSC · 42868-47070 · 4203 bp

Back to Centrolobium

Pi 0.0019
MarkerSeek score 54.80
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4203
variable_sites
8
pi
0.0019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 TCATAACGTGACTATTCCACCA CTGCACGACCTTCCTGATAA 40.9 50.0 56.9 57.3 264 bp 1.0 51.2
rpoC2_p2 ACGTGACTATTCCACCAAAAAG CTGCACGACCTTCCTGATAA 40.9 50.0 56.9 57.3 259 bp 1.0 51.1
rpoC2_p3 CGTGACTATTCCACCAAAAAGT CTGCACGACCTTCCTGATAA 40.9 50.0 56.9 57.3 258 bp 1.0 51.1
rpoC2_p4 AACGTGACTATTCCACCAAA CTGCACGACCTTCCTGATAA 40.0 50.0 54.9 57.3 260 bp 1.0 51.1
rpoC2_p5 ACGTGACTATTCCACCAAAA CTGCACGACCTTCCTGATAA 40.0 50.0 54.9 57.3 259 bp 1.0 51.1