Hotspot

ndhG

SSC · 122385-122915 · 531 bp

Back to Centrolobium

Pi 0.00753

MarkerSeek score 57.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 4
pi: 0.00753
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TCAACAGAAATAAATGTGGGA	CCCTGGTCGTATCAATTAAGGT	33.3	45.5	53.0	57.6	662 bp	1.0	40.5
ndhG_p2	TCAACAGAAATAAATGTGGGAA	CCCTGGTCGTATCAATTAAGGT	31.8	45.5	53.9	57.6	662 bp	1.0	40.5
ndhG_p3	TTCAACAGAAATAAATGTGGGA	CCCTGGTCGTATCAATTAAGGT	31.8	45.5	53.9	57.6	663 bp	1.0	40.5
ndhG_p4	TCAACAGAAATAAATGTGGGA	TCCCTGGTCGTATCAATTAAGG	33.3	45.5	53.0	57.3	663 bp	1.0	40.5
ndhG_p5	TCAACAGAAATAAATGTGGGAAA	CCCTGGTCGTATCAATTAAGGT	30.4	45.5	54.7	57.6	662 bp	1.0	40.5