Hotspot

ndhF-rpl32

SSC · 116501-117257 · 757 bp

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Pi 0.0106

MarkerSeek score 59.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 757
variable_sites: 8
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGTCCCACCCCTATCAACA	TGCTGTCCAATATCCCCTCT	50.0	50.0	57.9	57.8	919-920 bp	1.0	78.7
ndhF-rpl32_p2	AGTCCCACCCCTATCAACAT	TGCTGTCCAATATCCCCTCT	50.0	50.0	57.7	57.8	918-919 bp	1.0	76.8
ndhF-rpl32_p3	AAGTCCCACCCCTATCAACA	GTGCGTTTCTTTGGAACTGC	50.0	50.0	57.9	58.5	858-859 bp	1.0	76.1
ndhF-rpl32_p4	AAAGTAGAAGTCCCACCCCT	TGCTGTCCAATATCCCCTCT	50.0	50.0	57.6	57.8	926-927 bp	1.0	75.6
ndhF-rpl32_p5	AGTCCCACCCCTATCAACAT	GTGCGTTTCTTTGGAACTGC	50.0	50.0	57.7	58.5	857-858 bp	1.0	74.3