Hotspot

rpl16-rps3

LSC · 86935-87101 · 167 bp

Back to Centrolobium

Pi 0.018

MarkerSeek score 62.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 3
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAAAACCAACGAGTCGCACA	GAGGGTAGGGTTCCTCTACA	45.0	55.0	58.9	57.2	312 bp	1.0	66.7
rpl16-rps3_p2	CCAACGAGTCGCACACTAAG	GAGGGTAGGGTTCCTCTACA	55.0	55.0	58.9	57.2	307 bp	1.0	66.5
rpl16-rps3_p3	CGAGTCGCACACTAAGCAT	GAGGGTAGGGTTCCTCTACA	52.6	55.0	58.0	57.2	303 bp	1.0	65.8
rpl16-rps3_p4	AAAAACCAACGAGTCGCACA	GAGGGTAGGGTTCCTCTACAA	45.0	52.4	58.9	57.9	312 bp	1.0	64.6
rpl16-rps3_p5	CCAACGAGTCGCACACTAA	GAGGGTAGGGTTCCTCTACA	52.6	55.0	57.8	57.2	307 bp	1.0	64.5