Hotspot

rps11-rpl36

LSC · 82975-83506 · 532 bp

Back to Centrolobium

Pi 0.00202
MarkerSeek score 62.80
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
532
variable_sites
1
pi
0.00202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CGTCCATTTTTACGCGAACC AGGCGAGGGCGAATTATAGT 50.0 50.0 58.1 58.7 636-653 bp 1.0 77.8
rps11-rpl36_p2 TTACGCGAACCAATACGTCC AGGCGAGGGCGAATTATAGT 50.0 50.0 58.4 58.7 651-668 bp 1.0 75.9
rps11-rpl36_p3 CGTCCATTTTTACGCGAACC AAGGGCCTCTGTTCGTAAAA 50.0 45.0 58.1 57.1 682-699 bp 1.0 75.6
rps11-rpl36_p4 CGTCCATTTTTACGCGAACC GGCGAGGGCGAATTATAGTA 50.0 50.0 58.1 57.0 635-652 bp 1.0 74.9
rps11-rpl36_p5 CGTCCATTTTTACGCGAACC GTAGGCGAGGGCGAATTATA 50.0 50.0 58.1 57.0 638-655 bp 1.0 74.9