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psbE-petL

LSC · 67417-68785 · 1369 bp

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Pi 0.00589

MarkerSeek score 48.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1369
variable_sites: 8
pi: 0.00589
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1628-1633 bp	1.0	80.9
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1627-1632 bp	1.0	80.9
psbE-petL_p3	TGACAAATAACCAACCCGCA	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1525-1530 bp	1.0	80.9
psbE-petL_p4	AAAGGATCAAAACGGCCAGT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1630-1635 bp	1.0	80.8
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1629-1634 bp	1.0	80.8