Hotspot

petA-psbJ

LSC · 65536-66638 · 1103 bp

Back to Centrolobium

Pi 0.00544

MarkerSeek score 62.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 6
pi: 0.00544
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAGGTGCAATTGTCCGAG	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1164-1188 bp	1.0	74.4
petA-psbJ_p2	GAAGGTGCAATTGTCCGAGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1163-1187 bp	1.0	74.4
petA-psbJ_p3	GAGAAGGTGCAATTGTCCGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1165-1189 bp	1.0	74.4
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	1243-1267 bp	1.0	74.0
petA-psbJ_p5	AGGTGCAATTGTCCGAGATG	CATATTCCGGATTGGGCTCA	50.0	50.0	58.3	57.4	1161-1185 bp	1.0	73.9