Hotspot

trnQ-UUG-rps16

LSC · 57886-58492 · 607 bp

Back to Centrolobium

Pi 0.00659

MarkerSeek score 62.50

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 607
variable_sites: 4
pi: 0.00659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAATCAAAAATGGGGCGTGG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.0	58.0	745 bp	1.0	80.3
trnQ-UUG-rps16_p2	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	687 bp	1.0	79.8
trnQ-UUG-rps16_p3	GTCGAATCAAAAATGGGGCG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.1	58.0	748 bp	1.0	79.7
trnQ-UUG-rps16_p4	GAATCAAAAATGGGGCGTGG	ATGTCTTTCAAGTCGCACGT	50.0	45.0	58.0	58.1	747 bp	1.0	79.3
trnQ-UUG-rps16_p5	GAATCAAAAATGGGGCGTGG	GCACGTTGCTTTTTACCACA	50.0	45.0	58.0	57.8	733 bp	1.0	78.8