Hotspot

atpH-atpF

LSC · 50598-51064 · 467 bp

Back to Centrolobium

Pi 0.00905

MarkerSeek score 65.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 4
pi: 0.00905
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GGCTGGTTGTAGCATTAGCA	GATGGCCAGTAACCCAAAGA	50.0	50.0	58.2	57.5	549-561 bp	1.0	78.1
atpH-atpF_p2	GGCTGGTTGTAGCATTAGCA	GGATGGCCAGTAACCCAAAG	50.0	55.0	58.2	58.5	550-562 bp	1.0	78.0
atpH-atpF_p3	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACCCAAAGA	50.0	50.0	57.6	57.5	551-563 bp	1.0	76.8
atpH-atpF_p4	TGGGCTGGTTGTAGCATTAG	GGATGGCCAGTAACCCAAAG	50.0	55.0	57.6	58.5	552-564 bp	1.0	76.6
atpH-atpF_p5	AGGCAGAGGGAAAAATACGG	GATGGCCAGTAACCCAAAGA	50.0	50.0	57.6	57.5	618-630 bp	1.0	76.3