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psbM-petN

LSC · 32920-34225 · 1306 bp

Back to Centrolobium

Pi 0.00339

MarkerSeek score 64.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1306
variable_sites: 4
pi: 0.00339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.6	58.0	1334-1461 bp	1.0	72.9
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	57.6	57.8	1340-1467 bp	1.0	71.4
psbM-petN_p3	TGCTACTGCACTGTTCATTC	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	56.1	58.0	1334-1461 bp	1.0	68.7
psbM-petN_p4	TGCTACTGCACTGTTCATTC	AGTAAGTCTTGCTTGGGCTG	45.0	50.0	56.1	57.8	1340-1467 bp	1.0	67.3
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	TTGCTTGGGCTGCTTTAATG	42.9	45.0	57.6	57.3	1332-1459 bp	1.0	67.0