Hotspot

trnY-GUA-trnD-GUC

LSC · 31820-32337 · 518 bp

Back to Centrolobium

Pi 0.0106

MarkerSeek score 58.10

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 5
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	542-588 bp	1.0	79.0
trnY-GUA-trnD-GUC_p2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	541-587 bp	1.0	79.0
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	563-609 bp	1.0	78.1
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	562-608 bp	1.0	77.9
trnY-GUA-trnD-GUC_p5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	596-642 bp	1.0	77.5