Hotspot

psbZ-trnS-UGA

LSC · 26124-26477 · 354 bp

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Pi 0
MarkerSeek score 30.40
Primer available yes
Label psbZ-trnS-UGA

Feature Metadata

label_name
psbZ-trnS-UGA
feature_type
igs
region
LSC
length_bp
354
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnS-UGA_p1 CGACCAACCATCAGGAGAAG CAACCACTCAGCCATCTCTC 55.0 55.0 58.0 58.0 477 bp 1.0 79.7
psbZ-trnS-UGA_p2 CGACCAACCATCAGGAGAAG GAGCTATCAACCACTCAGCC 55.0 55.0 58.0 58.1 484 bp 1.0 79.4
psbZ-trnS-UGA_p3 CGACCAACCATCAGGAGAAG GGAGCTATCAACCACTCAGC 55.0 55.0 58.0 58.1 485 bp 1.0 79.4
psbZ-trnS-UGA_p4 CGACCAACCATCAGGAGAAG CAAGACCGGAGCTATCAACC 55.0 55.0 58.0 58.1 492 bp 1.0 79.4
psbZ-trnS-UGA_p5 CCAACCATCAGGAGAAGCAA CAACCACTCAGCCATCTCTC 50.0 55.0 57.8 58.0 474 bp 1.0 78.2